Protein_Domain

Part:BBa_K4181020:Design

Designed by: Junzhi Yan   Group: iGEM22_SJTU-BioX-Shanghai   (2022-10-05)


cotE→His tag→linker


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 298
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 298
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 303
    Illegal XhoI site found at 491
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 298
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 298
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1571


Design Notes

None


Source

atgtctgaatacagggaaattattacgaaggcagtagtagcgaaaggccgaaaattcacccaatgcaccaacaccatctcgcctgagaaaaaaccgagcagcattttgggtggttggattattaaccacaagtatgacgctgaaaaaattggaaaaacggtagaaattgaagggtattatgatataaacgtatggtactcttacgcggacaacacaaagacagaggttgtcacagaacgggtaaaatatgtagatgtcattaaactcagatacagagacaataattacttagatgatgagcatgaagtgattgccaaagtgcttcagcagccaaactgccttgaagtgaccatttcgccgaatggaaataaaatcgttgtgcaggcagaaagagaatttttggcggaagtggtaggggaaacaaaggtagttgttgaggtcaatcctgactgggaagaggatgacgaggaagattgggaagatgagcttgatgaagagcttgaagacatcaacccggagtttttagtgggagatcctgaagaataatggtggtggaggatct

References